Potentials of intravenous laser blood irradiation to correct peroxidation and antioxidant activity as a result of surgical distress

Purpose: to reveal protective effects of intravenous low-level laser blood irradiation (ILBI) during surgical interventions at the gallbladder as well as to study the state of antioxidant activity, lipid peroxidation and endogenous intoxication.

Material and methods. In the perioperative period, 40 patients from the main group (I) had traditional treatment which included pharmacological preparations and sessions of intravenous laser blood irradiation. 45 patients from the control group (II) had only traditional (pharmacotherapeutic) treatment. In both groups during surgery, the anesthetic protection consisted of classical neuroleptanalgesia. The age of patients in both groups did not differ significantly. Intravenous laser blood irradiation was done with helium-neon laser “Atoll” 632 nm (Fokon Ltd, St-Petersburg, Russia). During surgery, three irradiation sessions were performed at radiation power of 20 mW and exposure 15 minutes at various stages of surgery: 30 minutes before induction of anesthesia, at the traumatic stage and 30 minutes before the expected finish of the surgery. Ceruloplasmin and transferrin levels in the blood plasma were assessed with the electron paramagnetic resonance technique. The ratio of ceruloplasmin/transferrin which characterizes the level of antioxidant activity was also established. The level of endogenous intoxication was assessed using the previously published technique. The quantitative characteristics of endogenous intoxication by this technique is the value of optical density (D280) and total optical density of components related to high molecular weight and low molecular weight fractions.

Results. ILBI sessions in patients neutralize changes in the level of ceruloplasmin and transferrin in blood. The data obtained also confirm ILBI protective effects at protein SH-groups or activation of the restoration of protein SH-groups that were damaged by oxidation as a result of surgical aggression and trauma.

Conclusion. The protective effect of intravenous laser blood irradiation in addition to the standard preoperative therapy optimizes pharmacological anesthetic protection during surgery under the existing traditional schemes of combined general anesthesia.

1. Gaschler M.M., Stockwell B.R. Lipid peroxidation in cell death. Biochem Biophys Res Commun. 2017; 482 (3): 419–425. DOI: 10.1016/j.bbrc.2016.10.086

2. Shcherbak V.A. Bile lipid peroxidation in chronic gastroduodenitis in children. Actual problems of clinical and experimental medicine. 2018; 130–132. [In Russ.]

3. Kolesnikova L.I., Rychkova L.V., Kolesnikov S.I. The evaluation of lipid peroxidation system and antioxidant defense in adolescent boys with exogenously constitutive obesity with using the coefficient of oxidative stress. Voprosy pitaniya. 2018; 87 (1): 28–34. [In Russ.]. DOI: 10.24411/0042-8833-2018-10003

4. Kolesnikova L.I., Darenskaya M.A., Rychkova L.V., et al. Antioxidant status in adolescents of small Siberian ethnoses. J Evol Biochem Physiol. 2018; 54 (2): 116–121. [In Russ.]

5. Oksenyuk O.S., Kalmykova Yu.A., Smirnova O.B., et al. The role of oxidative stress in the development of chronic kidney diseases and ways of its evaluation. Zhurnal fundamental’noy meditsiny i biologii. 2016; (1): 15–24. [In Russ.]

6. Razina A.O., Runenko S.D., Achkasov E.E. Obesity problems: Current trends in Russia and in the world. Vestnik Rossiiskoi akademii medetsinskikh nauk (Annals of the Russian Academy of Medical Sciences). 2016; 71 (2): 154–159. [In Russ.]. DOI: 10.15690/vramn655

7. Sumin S.A., Avdeeva N.N., Bystrova N.A., et al. Structural and functional properties of erythrocytes when using various methods of multicomponent general anesthesia in laparoscopic cholecystectomy in patients with cholelithiasis. Anesteziologiya i reanimatologiya (Russian Journal of Anesthesiology and Reanimatology). 2016; 4 (61): 296–300. [In Russ.]. DOI: 10.18821/0201-7563-2016-61-4-296-300

8. Malyshev V.D., Potapov A.F. Disorders of lipid peroxidation processes in surgical patients at the stages of treatment. Anesteziologiya i reanimatologiya (Russian Journal of Anesthesiology and Reanimatology). 1994; (6): 53–59. [In Russ.]

9. Zhuravlev A.I., Zubkova S.M. Antioxidants Free radical pathology. Moscow; 2008: 269 p. [In Russ.]

10. Fucho R., Casals N., Serra D., Herrero L. Ceramides and mitochondrial fatty acid oxidation in obesity. FASEB J. 2017; 31 (4): 1263–1272. DOI: 10.1096/fj.201601156R

11. Ryabov G.A., Azizov Yu.M., Pasechnik I.N., Krylov V.V., Tsvetkov D.S. Oxidative stress and endogenous intoxication in critically ill patients. Vestnik intensivnoy terapii. 2002; (4): 4–7. [In Russ.].

12. Smolnikov P.V., Shiriajev V.S., Musikhin L.V., et al. Low-intensive laser therapy in the geriatric practice of anaesthesiologists-reanimatologists. Laser Medicine. 2015; 19(4): 11–18. [In Russ.]. DOI: 10.37895/2071-8004-2015-19-4-11-18