Effects of photodynamic therapy with a photosensitizer complexed with amphiphilic polymers at reparative processes in experimental gunshot wounds

Purpose: To assess the effectiveness of photodynamic therapy (PDT) with photosensitizer Photoditazine complexed with amphiphilic polymers in the treatment of experimental gunshot wounds of soft tissues. Material and methods. Experimental studies were conducted on 50 non-linear rats, divided into 3 groups, depending on the curative modality: controls – traditional treatment; experimental Group 1 – PDT with Photoditazine in the form of 0.5% aqueous solution; experimental Group 2 – PDT with the complex of microencapsulated Photoditazine 0.1% – amphiphilic polymer. To treat wounds with PDT, AKTUS-2 device of Russian production was used. Wavelength of laser irradiation 661 ± 0.03 nm; power density 1 W/cm2 ; energy density 50 J/cm2 . Findings obtained after cytological and histological examinations were analyzed by the authors. Results. In 10 days after PDT, smears showed active proliferation as well as differentiation of reparation elements in Group 2. The increase of mononuclear elements up to 9.4 ± 0.8% and of the total number of young and mature fibroblasts were noted as well. If to compare these parameters with previous comparison groups, wound exudate contained twice more cellular reparation elements, and there were no degeneratively altered neutrophils. PDT with Photoditazine complexed with amphiphilic polymers stops disorders in the microcirculation system and activates the alternative-exudative phase of inflammation. Moreover, in the concussion zone, development of secondary necroses is limited, and the viability of damaged tissues is restored. Conclusion. PDT with a complex of microencapsulated Photoditazine and amphiphilic polymer leads to a quick restoration of viability in damaged tissues in the concussion zone, to early development of reparation processes, to expressed macrophage activation, to stimulation of angiogenesis and collagenogenesis as well as to the accelerated scarring and epithelization of wound defects in experimental gunshot wounds of soft tissues.

photodynamic therapy, purulent wound, wound microflora, photosensitizer

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