Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis
https://doi.org/10.37895/2071-8004-2021-25-2-41-47
Abstract
Purpose: To find out a technique for determining the effectiveness of porphyrin-type photosensitizers with concentrations which cause hemolysis in erythrocytes (hemolysis threshold). The hemolysis threshold is found under the following conditions: irradiation in the Soret band with standard parameters – power density, exposure dose, irradiation time.
Material and methods. Phototherapeutic device “AST” (LLC “Pankov-medical”) – average power 0.5 W, wavelength ≈ 405 nm. Rat blood erythrocytes. “Multiscan MS” device manufactured by Labsystems, Finland. Preparation “Dimegin”, hematoporphyrin derivative; preparation “Photoditazine” (LLC “VETA-GRAND”), chlorine E6 derivative.
Results. On analyzing the results obtained after experimentation with the abovementioned two preparations, it was shown that the developed technique has a high efficacy. It was also shown that the studied photosensitizers are equally effective when irradiated in the Soret band.
Conclusion. The developed technique is simple and easy to use. It helps to assess the efficacy of various photosensitizers thus, facilitating the selection of their necessary concentrations for managing different diseases.
About the Authors
Yu. V. Alekseev
Skobelkin State Scientific Center of Laser Medicine
Russian Federation
Russian Federation
Alekseev Yuriy – Dr. Sci. (Med.), Head of the Department of Experimental Laser Medicine
Moscow
O. v Mislavsky
Skobelkin State Scientific Center of Laser Medicine; National Research Center – Institute of Immunology
Russian Federation
Russian Federation
Mislavsky Oleg – Cand. Sci. (Pharm.), Senior Researcher at the Laboratory of Molecular Mechanisms of Allergy; Senior Researcher at the Department of Experimental Laser Medicine
Moscow
V. A. Duvanskiy
Skobelkin State Scientific Center of Laser Medicine; Peoples’ Friendship University of Russia
Russian Federation
Russian Federation
Duvanskiy Vladimir – Dr. Sci. (Med.), Professor, Deputy Director; Head of the Department of Endoscopy, Endoscopic and Laser Surgery
Moscow
R. A. Duvanskiy
Skobelkin State Scientific Center of Laser Medicine; Treatment and Rehabilitation Center of National Medical Research Center
Russian Federation
Russian Federation
Duvanskiy Roman – Cand. Sci. (Med.), Senior Researcher at the Department of Endoscopic Surgery; Obstetrician-Gynecologist, Treatment and Rehabilitation Center of National Medical Research Center
Moscow
N. V. Drozdova
Skobelkin State Scientific Center of Laser Medicine
Russian Federation
Russian Federation
Drozdova Natalya – Junior Researcher at the Department of Experimental Laser Medicine
Moscow
References
1. Dadeko A.V. Photophysical properties of Dimegin as a photosensitizer for photodynamic therapy and fluorescence diagnostics. Abstract of the dissertation thesis of Candidate of Physical and Mathematical Sciences. Saint Petersburg; 2018. [In Russ.].
2. Kozel N.V., Shalygo N.V. A technique for assessing the photosensitizer phototoxicity. Patent for invention BY 15504 C1, 28.02.2012. Application N 20081283 dated 13.10.2008. [In Russ.].
3. Yakubovskaya R.I., Plyutinskaya A.D., Lukyanets E.A. Comparative study of photosensitizers of different classes in an in vitro system. Phthalocyanines. Rossiyskiy bioterapevticheskiy zhurnal. 2014. 13 (4): 65–72. [In Russ.].
4. Makarova E.A., Yakubovskaya R.I., Vorozhtsov G.N., Lastovoy A.P., Lukyanets E.A., Morozova N.B., Plotnikova E.A. Photosensitizer for photodynamic therapy. Patent for invention RU 2549953 C2, 05.10.2015. Application N 2013157611/15 dated 25.12.2013. [In Russ.].
5. Bondarenko V.M., Alekseev Yu.V., Mislavsky O.V., Ponomarev G.V. Prospects for the application of 2,4-di(1- methoxyethyl)-deuteroporphyrin-IX (“dimegin”) disodium salt for photodynamic therapy of non-oncological diseases. Biomeditsinskaya khimiya. 2014; 60 (3): 338–347. [In Russ.].
6. Mishchenko Т.А., Mitroshina Е.V., Turubanova V.D., Alzeibak R., Balalaeva I.V., Vedunova М.V., et al. Effect of photosensitizers Photosens, Photodithazine and Hypericin on glioma сells and primary neuronal cultures: A comparative analysis. Modern Technologies in Medicine. 2019; 11 (4): 52–63. DOI: 10.17691/stm2019.11.4.06
7. Jori G., Brown S.B. Photosensitized inactivation of microorganisms. Photochem Photobiol Sci. 2004; (3): 403–405. DOI: 10.1039/b311904c
8. Nitzan Y., Gutterman М., Malik Z., Ehrenberg В. Inactivation of gram-negative bacteria by photosensitized porphyrins. Photochem Photobiol. 1992; 55 (1): 89–96. DOI: 10.1111/j.1751-1097.1992. tb04213.x
9. Plotnikova E.A., Grin M.A., Ostroverkhov P.V., Pantyuchenko I.V., Yakubovskaya R.I., Kaprin A.D. Primary screening of substances-photosensibilizers of the bacteriochlorin range
10. for photodynamic therapy of malignant neoplasms. Biomedicinskaya himiya. 2018; 64 (3): 283–289. DOI: 10.18097/PBMC20186403283. [In Russ.].
11. Stranadko E.F. Main stages of development of photodynamic therapy in Russia. Photodynamic therapy and photodyagnosis. 2015; 4 (1): 3–10. DOI: 10.24931/2413-9432-2015-4-1-3-
12. [In Russ.].
13. Filonenko E.V., Serova L.G. Photodynamic therapy in clinical practice. Biomedical Photonics. 2016; 5 (2): 26–37. [In Russ.].
14. Duvansky V.A., Popova E.A. The first experience with photodynamic therapy in the complex treatment of duodenal ulcers. Lazernaya medicina. 2004; 8 (3): 217. [In Russ.].
15. Abrahamse H., Hamblin M.R. New photosensitizers for photodynamic therapy. Biochem J. 2016; 473(4): 347–364. DOI: 10.1042/BJ20150942
16. Douvansky V.A., Kniazev M.В. Autofluorescent endoscopic diagnostics of epithelial neoplasms in the colon. Journal of Gastroenterology and Hepatology. 2015; 30 (4): 211.
17. Knyazev M.V., Duvansky V.A. Autofluorescent endoscopy in the diagnosis of colorectal neoplasia. Endoskopicheskaya khirurgiya. 2014; (1): 186. [In Russ.].
18. Makarova Yu.B. Diagnostics of chronic inflammatory skin diseases and assessment of the effectiveness of their care with the technique of laser biophotometry. Abstract of Dissertation Thesis of Candidate of Medical Sciences. Tula; 2007. [In Russ.].
19. Alekseev Yu.V., Likhacheva E.V., Tereshkin D.V., Ponomarev G.V., Mazur E.M. Selection of effective photosensitizers for the treatment of ENT diseases with studying their accumulation in pathologically altered tissues. Biomeditsinskaya khimiya. 2012; 58 (1): 112–120. DOI: 10.18097/pbmc20125801112. [In Russ.].
20. Mansurova G.V., Pogrebnaya O.G., Ponomarev G.V., Reshetnikov A.V., Potapenko A.Ya., Bezdetnaya L.N., et al. Photohemolysis sensitized by deuteroporphyrin-IX derivatives: Strength of binding of dyes with erythrocytes. Biofizika. 2003; 48 (2): 251–255. [In Russ.].
21. Larina N.A. Hemolysis of human erythrocytes induced by the products of furacumarin photooxidation and suppression of contact sensitivity response in mice. Dissertation Thesis of Candidate of Medical Sciences. Moscow; 2001: 23–35. [In Russ.].
22. Cook J.C. Some characteristics of hemolysis by ultraviolet light. J Cell Comp Physiol. 1956; 47 (1): 55–84. DOI: 10.1002/jcp.1030470105
23. Mansurova G.V. Screening of hemolytic and immunosuppressive activity of photosensitizers of the porphyrin series. Abstract of Dissertation Thesis of Candidate of Biological Sciences. Moscow; 2007. [In Russ.].
24. Alekseev Yu.V., Mislavsky O.V., Ponomarev G.V., Ivanov A.V., Davydov E.V. A technique for determining the efficiency of photosensitizers and parameters of laser 1264 nm irradiation using erythrocyte hemolysis thresholds. Proceedings of the International Conference “Lasers in science, technology and medicine”. Moscow; 2020: 106–107. [In Russ.].
Review
For citations:
Alekseev Yu.V., Mislavsky O.v., Duvanskiy V.A., Duvanskiy R.A., Drozdova N.V. Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis. Laser Medicine. 2021;25(2):41-47. (In Russ.) https://doi.org/10.37895/2071-8004-2021-25-2-41-47
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