Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis
https://doi.org/10.37895/2071-8004-2021-25-2-41-47
Abstract
Purpose: To find out a technique for determining the effectiveness of porphyrin-type photosensitizers with concentrations which cause hemolysis in erythrocytes (hemolysis threshold). The hemolysis threshold is found under the following conditions: irradiation in the Soret band with standard parameters – power density, exposure dose, irradiation time.
Material and methods. Phototherapeutic device “AST” (LLC “Pankov-medical”) – average power 0.5 W, wavelength ≈ 405 nm. Rat blood erythrocytes. “Multiscan MS” device manufactured by Labsystems, Finland. Preparation “Dimegin”, hematoporphyrin derivative; preparation “Photoditazine” (LLC “VETA-GRAND”), chlorine E6 derivative.
Results. On analyzing the results obtained after experimentation with the abovementioned two preparations, it was shown that the developed technique has a high efficacy. It was also shown that the studied photosensitizers are equally effective when irradiated in the Soret band.
Conclusion. The developed technique is simple and easy to use. It helps to assess the efficacy of various photosensitizers thus, facilitating the selection of their necessary concentrations for managing different diseases.
About the Authors
Yu. V. Alekseev
Skobelkin State Scientific Center of Laser Medicine
Russian Federation
Russian Federation
Alekseev Yuriy – Dr. Sci. (Med.), Head of the Department of Experimental Laser Medicine
Moscow
O. v Mislavsky
Skobelkin State Scientific Center of Laser Medicine; National Research Center – Institute of Immunology
Russian Federation
Russian Federation
Mislavsky Oleg – Cand. Sci. (Pharm.), Senior Researcher at the Laboratory of Molecular Mechanisms of Allergy; Senior Researcher at the Department of Experimental Laser Medicine
Moscow
V. A. Duvanskiy
Skobelkin State Scientific Center of Laser Medicine; Peoples’ Friendship University of Russia
Russian Federation
Russian Federation
Duvanskiy Vladimir – Dr. Sci. (Med.), Professor, Deputy Director; Head of the Department of Endoscopy, Endoscopic and Laser Surgery
Moscow
R. A. Duvanskiy
Skobelkin State Scientific Center of Laser Medicine; Treatment and Rehabilitation Center of National Medical Research Center
Russian Federation
Russian Federation
Duvanskiy Roman – Cand. Sci. (Med.), Senior Researcher at the Department of Endoscopic Surgery; Obstetrician-Gynecologist, Treatment and Rehabilitation Center of National Medical Research Center
Moscow
N. V. Drozdova
Skobelkin State Scientific Center of Laser Medicine
Russian Federation
Russian Federation
Drozdova Natalya – Junior Researcher at the Department of Experimental Laser Medicine
Moscow
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Review
For citations:
Alekseev Yu.V., Mislavsky O.v., Duvanskiy V.A., Duvanskiy R.A., Drozdova N.V. Assessment of the photosensitizer efficacy after determination of threshold doses initiating erythrocyte hemolysis. Laser Medicine. 2021;25(2):41-47. (In Russ.) https://doi.org/10.37895/2071-8004-2021-25-2-41-47
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