Features of response reactions of some cell populations of connective tissue and collagen formation in a rat model with postoperative ventral hernia after laser irradiation on red bone marrow

Prevention of postoperative hernias of the anterior abdominal wall is an actual problem of abdominal surgery. Impairments in the quantitative ratio of collagens of types I/III negatively affect characteristics of the forming connective tissue what leads to the formation of hernia recurrences. Purpose. To study effects of laser light irradiation of bone marrow location zones at the number and ratio of collagen types I and III, at the number of fibroblasts, at the area of vascular bed and morphofunctional activity of mast cells in the forming connective tissue scar. Material and methods. 20 laboratory rats with postoperative ventral hernia were taken into the experiment. They were divided into two groups: control and experimental. Zones of bone marrow in experimental animals were irradiated with laser light (980 nm, power 1.0 W, continuous mode, 300 s, five sessions ). In 30 days after hernia modeling, tissue samples were fixed in formalin; histological sections were prepared; area of vascular flow, level of fibroblasts, mast cells, and their morphofunctional activity were studied with the morphometric method. The level of collagens of type I and III was studied with the immunohistochemical method. Results. Laser exposure to bone marrow significantly increased type I collagen level and ratio of type I and III collagen; it also increases the number of fibroblasts, area of vascular bed, morphofunctional activity of mast cells in tissues of the anterior abdominal wall in rats with a model of postoperative ventral hernia. These promising findings may promote further researches on developing a technique for preventing hernia formation.

laser irradiation, hernia, collagen, fibroblasts, mast cells, blood vessels

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