Morphofunctional properties of human platelets after laser irradiation in situ
https://doi.org/10.37895/2071-8004-2019-23-2-26-31
Abstract
Purpose. To study morphofunctional characteristics of human platelets irradiated in situ with modulated light of the visible spectrum. Material and methods. Donor platelets were harvested by the automatic apheresis, stained with vital dye and irradiated with a laser system attached to the confocal microscope «Nikon D-Eclipse C1» (Nikon, Japan). Modulated light with long UV-wave (λ = 408 nm), blue light (λ = 488 nm), green light (λ = 543 nm) and red light (λ = 637 nm) was used for the irradiation; irradiation sessions lasted for 0.5–60 min. Results. Аfter low-dose light irradiation with λ = 488 nm and λ = 543, human platelets did not have any visible changes; after irradiation with red light (λ = 637 nm), platelets with normal biological status were quickly degranulated and activated; degranulation intensity depended on the irradiation time. A similar effect was registered during long wave UV-irradiation (λ = 408 nm), while the followed platelet activation was lower than after red light irradiation. Granules offset to the cytoplasm periphery started in 0.5–1 min after red irradiation and in 10 minutes after ultraviolet one. Total activation of biologically high-grade platelets was seen in 30 and 60 min, respectively. Conclusion. Еxposure of human platelets to red and ultraviolet light in situ stimulates their spontaneous activation without any damage to the platelet structure.
About the Authors
M. S. Makarov
Sklifosovsky Research Institute of Emergency Care
Russian Federation
Russian Federation
V. B. Khvatov
Sklifosovsky Research Institute of Emergency Care
Russian Federation
Russian Federation
References
1. Gorbatenko E.A., Azizova O.A., Vladimirov Yu.A. Reactivation of superoxide dismutase by helium-neon laser radiation. Biofizika. 1988; 33: 717–718. [In Russ.].
2. Kozlov V.I. Photobiostimulation mechanisms. Lasernaya medicina. 2010; 14 (4): 4–13. [In Russ.].
3. Kozlov V.I., Litvin F.B., Ryzhakin S.M. Effects of radiation with helium-neon laser light at microcirculatory vessels in the pia mater of the brain. Lasernaya medicina. 2002; 6 (2): 22–24. [In Russ.].
4. Loiko E.N., SamalA.B., Shulyakovskaya S.M. H2O2-induced platelet aggregation and increase of Ca2 + ions intracellular concentration are blocked by inhibitors of intracellular signaling. Biochimia. 2003; 68 (11): 1506–1510. [In Russ.].
5. Makarov M.S., Khvatov V.B., Kobzeva E.N. et al. Morphofunctional analysis of human platelets using vital staining. Bulletin experimentalnoy biologii i medicini. 2013; 9: 388–391. [In Russ.].
6. Makarov M.S. Monitoring of morphofunctional status of human platelets under oxidative stress induced by hydrogen peroxide. Meditzinsky alphavit. Sovremennaya laboratoria. 2017; 3(26): 38–39. [In Russ.].
7. Marchenko A.V., Dutkevich I.G., Musalov M.A. Photohemotherapy in the complex treatment of peptic ulcer. Efferentnaya therapia.1995; 1(2): 65–67. [In Russ.].
8. Moskvin S.V., Fedorova T.A., Foteeva T.S. Plasmapheresis and laser blood screening. M.–Tver: Triada, 2018: 416. [In Russ.].
9. Optical biomedical diagnostics. In 2 Volumes. Vol. 1 / translated from English by Tuchina. Moscow, FIZMATLIT, 2017: 560. [In Russ.].
10. Petrishchev N.N., Zubov B.V., Dementieva I.N. Comparative study of the effect of modulated LED irradiation of blood (630 nm, 450 nm) on platelet aggregation activity. Lasernaya medicina. 2011; 14 (3): 49–52. [In Russ.].
11. Ulashchik V.S. Photodynamic therapy – technology of the XXI century. Fizioterapia, balneologia i reabilitatzia. 2013; 1: 36–43. [In Russ.].
12. Efferent therapy. ed. A.L. Kostyuchenko. St-Petersburg: Foliant. 2003, 432. [In Russ.].
13. Breitbart H., Levinshal T., Cohen N. et al. Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser). J. of Photochemistry and Photobiology B. 1996; 34 (2–3): 117–121.
14. Freitag J., Barnard A., Rotstein A. Photoactivated platelet-rich plasma therapy for a traumatic knee chondral lesion. BMJ Case Rep. 2012; Р. bcr2012006858.
15. Løvschall H., Arenholt-Bindslev D. Effect of low level diode laser irradiation of human oral mucosa fibroblasts in vitro. Lasers in Surgery and Medicine. 1994; 14 (4): 347–354.
16. Lubart R., Friedmann H., Sinyakov M. et al. The effect of HeNe laser (633 nm) radiation on intracellular Cа2+ concentration in fibroblasts. Laser Therapy. 1997 (1); 9 (3): 115–120.
17. Paterson K.L., Nicholls M., Bennell K.L. et al. Intra-articular injection of photo-activated platelet-rich plasma in patients with knee osteoarthritis: a double-blind, randomized controlled pilot study. BMC Musculoskelet Disord. 2016; 17: 67.
18. Zhevago N.A., Samoilova K.A. Pro- and anti-inflammatory cytokine content in human peripheral blood after its transcutaneous (in vivo) and direct (in vitro) irradiation with polychromatic visible and infrared light. Photomed Laser Ther. 2006; 24 (2): 129–139.
Review
For citations:
Makarov M.S., Khvatov V.B. Morphofunctional properties of human platelets after laser irradiation in situ. Laser Medicine. 2019;23(2):26-31. (In Russ.) https://doi.org/10.37895/2071-8004-2019-23-2-26-31
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