Development of a technique for treating widespread purulent peritonitis (an experimental study)
https://doi.org/10.37895/2071-8004-2019-23-1-46-52
Abstract
Purpose: To develop a new technique for surgical treatment of purulent peritonitis.
Material and methods. 100 Wistar rats were divided into 3 groups and were operated on. Peritonitis was modulated and then, on day 2, enteric anastomosis was formed at 90° and 60° angles of intestinal resection. On day 7, the animals were taken out of the experiment. Laser photodynamic therapy (PDT) was applied to the formed anastomosis using semiconductor laser Lakhta Milon with wavelength 662 nm and output power 200 mW. The targeted area was irradiated with a distant mono position mode to achieve light dose density equal to 20–25 J/cm2. 0.3% methylene blue solution was used as a photosensitizer. Postoperative mortality, number of inconsistent anastomoses and abdominal abscesses were compared in the groups. The researchers also investigated blood and lymphatic vessels in the intestinal wall, level of neutrophilic leukocytes, lymphocytes and fibroblasts along the line of intestinal suture.
Results. As it has been found out, the number of inconsistent anastomoses and abdominal abscesses as well the mortality rate were the smallest in the group of animals in which anastomoses were made at 60° angle and then treated with photodynamic sessions. It was also noted that photodynamic therapy decreased the intensity of local inflammatory response in the intestinal wall.
Conclusion. The developed technique for intestinal anastomosis formation with the resection of both intestinal loops at the angle of 60° and the subsequent photodynamic treatment of anastomoses reduces the risk of their failure and abdominal abscesses.
About the Authors
J. S. Weiner
Novosibirsk State Medical University
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
S. D. Nikonov
Novosibirsk State National Research University (NSU)
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
K. V. Atamanov
Novosibirsk State Medical University
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
D. K. Atamanov
Novosibirsk State Medical University
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
E. V. Fedorova
Novosibirsk State Medical University
Russian Federation
Novosibirsk
Russian Federation
Novosibirsk
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Review
For citations:
Weiner J.S., Nikonov S.D., Atamanov K.V., Atamanov D.K., Fedorova E.V. Development of a technique for treating widespread purulent peritonitis (an experimental study). Laser Medicine. 2019;23(1):46-52. (In Russ.) https://doi.org/10.37895/2071-8004-2019-23-1-46-52
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