Effects of photosensitizer Radahlorine and photodynamic effects at the collagen-induced platelet aggregation

Purpose. To study the impact of photosensitizer Radahlorine and photodynamic effects (in vitro and in vivo) at the platelet aggregation activity. Materials and methods. Wistar rats (males) were used in the trial. Platelet-rich plasma (PRP) was taken from the venous blood. Aggregation activity of platelets (Pl) was measured with the turbodimetric technique (analyzer AT-02, Russia), aggregating inductor - collagen (Chrono-Log Corporation, USA) 4 ng/ml. Radahlorine (RH) (Radapharma, Russia) was used as a photosensitizer (PS). PRP samples with PS were irradiated with laser device ALOD-Granat (Alcom Medica, Russia) with wavelength 662 nm, power density - 0.05 W/cm², energy density - 12.5 J/cm², 25 J/cm², 50 J/cm². Results. Dose-depending Pl aggregation inhibition was observed after 5-minute incubation of PRP with RH (10, 20, 40 ng/ml). PRP irradiation (25 J/cm², 50 J/cm²) in presence of RH (14 ng/ml) significantly increased an inhibitory effect. Under the dose equal to 50 J/cm², latent period and PL shape-changing phase after Pl stimulation by collagen were absent, and the aggregation intensity decreased by 3 times. In 3 hours after RH (5ng/ml) intravenous administration, intensity of Pl aggregation did not change significantly, but after PRP irradiation with 25 J/cm² it decreased by 5 times. Conclusion. Radachlorine has a dose-depending inhibition effect at collagen-induced Pl aggregation in rats. Photoactivation increases this effect. One of the probable mechanisms of this effect is photodynamic damage of Pl membrane and impaired activation of glycoprotein receptors responsible for Pl aggregation.

Радахлорин, фотоактивация, коллаген, агрегация тромбоцитов, Radahlorine, photoactivation, collagen, platelet aggregation

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