Dynamics of some parameters of the cytokine profile in exudative pleuritis of various etiology under intravenous laser blood irradiation (interim results)

Objective. To study the dynamics of IL-1β, IL-4 and TNF-α levels in patients with pleural effusion of various etiology under lowintensive laser therapy (LILT). Material and methods. 68 patients with pleural effusion of various etiology were examined; 42 men (61.8%) and 26 women (38.2%). The average age was 67.6 + 13.9. All patients were randomly divided into two groups: control group (n = 30) and studied (n = 38). Depending on the etiology of pleural effusion, patients of both groups were divided into three subgroups. Healthy donors in a comparison group (n = 15) were comparable by sex and age. Patients from the control group had only drug therapy according to the etiology of pleural effusion. Patients of the studied group had additionally intravenous laser blood irradiation (ILBI). Levels of interleukin 1 (IL-1β), interleukin 4 (IL-4) and tumor necrosis factor-alpha (TNF-α) in blood serum was tested in all patients before therapy on days 1 or 2 after hospitalization and in 10-14 days after therapy onset. To define cytokine levels, test-systems manufactured by ZAO Vector-Best with solid-phase enzyme-linked immunosorbent assay and Victor2 immunoassay analyzer (PerkinElmer firm) were used. Laser therapy technique developed by Stogovaya was performed with Matrix-VLOK (Matrix, Russia) laser device (wavelength 365 nm, output power 1-1.5 mW). For intravenous laser blood irradiation (ILBI), puncture of the ulnar vein with a disposable needle and KIVL-01 light guide was made. Irradiation lasted for 10 minutes in a continuous mode. Laser therapy course had 10 sessions. Results and discussion. IL-1β level before treatment was increased in both groups in all patients compared to healthy subjects, regardless of pleural effusion etiology. After treatment, TNF-α level significantly decreased only in the studied group. One could also see a significant increase of IL-4 level in the studied group. There was no reliable dynamics of IL-4 level in the control group. Conclusion. If LILT is included into the complex therapy of patients with pleural effusions of various etiology, one can see a correction of imbalance between pro-inflammatory and antiinflammatory cytokines, what indicates that LILT has anti-inflammatory effect. So, laser therapy may be recommended for the complex treatment of patients with pleural effusions of various etiology.

плевральная жидкость, лазерная терапия, цитокиновый профиль, pleural fluid, laser therapy, cytokine profile

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