Morphofunctional changes in laser-destructed foci in the liver, kidney, spleen (an experimental study)

Abstract

A comparative study of morphological and functional changes in laser-destructed foci in the liver, kidneys and spleen was performed in Chelyabinsk State Institute of Laser Surgery. High-level continuous laser radiation was used. Contact laser destruction of the liver, kidneys, spleen was done from the surface of the organ in the contact mode in 20 rats. Radiation was continuous with wavelength 0.97 μm, power 2 W, exposure time 10 sec. Result were assessed in one hour and one day after. Histological, morphometric and statistical methods were used for this. Destructed area was measured; the number of mast cells was counted (total, intact - I, degranulated - D). The index of degranulation (ID) was determined as: ID = D / (D + I). It was found out that the foci destructed by laser light were cup-shaped in all investigated organs; they had zonal structure and comparable values of the total width. Functional activity of mast cells in lesions was increased in the liver and kidneys; there was also an increased number of mast cells and increased level of degranulation, what demonstrated a high rate of tissue reparation. In case of spleen resection, there was no such effects. Mast cells are likely not to play a leading role in this organ reparation.