Experimental substantiation of the selectivity effect of «green» laser light at hemoglobin-containing tissues
N. E. Gorbatova,
D. A. Safin,
E. N. Gasanova,
S. A. Zolotov,
A. A. Sirotkin,
G. P. Kuzmin,
A. S. Tertychny,
N. V. Stankova,
M. V. Remennicova,
O. V. Tikhonevich https://doi.org/10.37895/2071-8004-2020-24-2-3-95-103
Abstract
Currently, medical specialists still face problems while treating pathological subepithelial capillary structures, in particular, capillary angiodysplasias of the skin. One of the promising techniques for their removal is photothermolysis by laser light of yellow-green spectral range which is selectively absorbed by pigments, hemoglobin, blood in the vessels with further vessel hardening and regression. Purpose. To make a comparative trial in vitro at experimental models so as to confirm the effect of selectivity of «green» laser light at hemoglobin-containing tissues, in particular, at subepithelial vascular structures. Material and methods: An experimental solid-state semiconductor laser, generating green light with wavelength 525 nm which is near the absorption peak of hemoglobin and oxyhemoglobin, was used. Experimental models were cooled samples of the liver, skin and a preparation combined of them as well as laboratory minipigs of Svetlogorsk population. In the trial, the researchers compared changes developed after the irradiation of abovementioned objects with «green» laser light. To make comparison, morphological, macroscopic and histological findings from irradiated zones were analyzed. Results. Specific differences in the damaged zones of experimental models as well as in the liver and skin were found. While examining the liver, one can see locally more pronounced changes and a minus destruction looking like a narrow sphenoid-shaped crater with relatively narrow zones of compact and cellular necrosis, clear boundaries between them as well as unchanged adjacent tissues. On the skin, one can see a minus destruction of much smaller size with zone of peripheral coagulation 3–5 times wider and without compact and cellular layers; there are also no clear boundaries with unchanged tissues. In the combined sample, green laser light – which is less absorbed by the skin- penetrates through it, thus affecting the underlying liver where one can see more pronounced thermal changes, if to compare with the skin. Conclusion. The selectivity effect of «green» laser light with wavelength 525 nm at hemoglobin-containing tissues has been confirmed. This conclusion is a promising finding for applying the selective photothermolysis in pathological subepithelial capillary structures which will ensure precise treatment with minimal damage to skin tissues.
About the Authors
N. E. Gorbatova
Clinical and Research Institute of Emergency Pediatric Surgery and Trauma; National Medical Research Center of Children's Health
Russian Federation
Russian Federation
Gorbatova Natalya – MD, Dr.Sc. (med), academician of Academy of Medico-Technical Sciences of Russia, leading researcher, pediatric surgeon at Clinical and Research Institute of Emergency Pediatric Surgery and Trauma (CRIEPST), deputy head of Center for Pediatric Laser Surgery; senior researcher at National Center of Children's Health (NCZD). SPIN code: 6016-6891, Author ID: 300655
Moscow
D. A. Safin
Clinical and Research Institute of Emergency Pediatric Surgery and Trauma
Russian Federation
Russian Federation
Safin Dinar – MD, pediatric surgeon, postgraduate student
Moscow
E. N. Gasanova
Clinical and Research Institute of Emergency Pediatric Surgery and Trauma
Russian Federation
Russian Federation
Hasanova Ella – MD, pediatric surgeon, researcher
Moscow
S. A. Zolotov
Clinical and Research Institute of Emergency Pediatric Surgery and Trauma
Russian Federation
Russian Federation
Zolotov Sergey – MD, Cand. Sc. (med), senior researcher
Moscow
A. A. Sirotkin
Prokhorov General Physics Institute
Russian Federation
Russian Federation
Sirotkin Anatoly – Doctor of Physics and Mathematics, leading researcher at laboratory of physics of crystal growth
Moscow
G. P. Kuzmin
Prokhorov General Physics Institute
Russian Federation
Russian Federation
Kuzmin Gennady – Doctor of Physics and Mathematics, professor, head of laboratory of resonant interactions
Moscow
A. S. Tertychny
Sechenov First Moscow State Medical University
Russian Federation
Russian Federation
Tertychny Alexander – MD, Dr.Sc. (med), professor, pathologist, head of laboratory of electron microscopy and immunohistochemistry
Moscow
N. V. Stankova
Research Center of Biomedical Technologies
Russian Federation
Russian Federation
Stankova Natalia – Cand. Sc. (biology), head of laboratory of sports medicine and extreme states
Svetlye Gory, Moscow Region
M. V. Remennicova
Perm Federal Research Center, PAO Perm Scientific and Instrument-Making Company
Russian Federation
Russian Federation
Remennikova Maria – head of laboratory of medical lasers, junior researcher, laboratory of agrobiophotonics
Perm
O. V. Tikhonevich
Prokhorov General Physics Institute
Russian Federation
Russian Federation
Tikhonevich Oleg – engineer-physicist, researcher
Moscow
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Review
For citations:
Gorbatova N.E., Safin D.A., Gasanova E.N., Zolotov S.A., Sirotkin A.A., Kuzmin G.P., Tertychny A.S., Stankova N.V., Remennicova M.V., Tikhonevich O.V. Experimental substantiation of the selectivity effect of «green» laser light at hemoglobin-containing tissues. Laser Medicine. 2020;24(2-3):95-103. (In Russ.) https://doi.org/10.37895/2071-8004-2020-24-2-3-95-103
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