The model of the formation of an optoacoustic signal from erythrocytes for a laser cytometer

Abstract

Objective: to develop a two-dimensional model of red blood cell distribution in plasma for 200 species of location of non-aggregated red blood cells (tissue models) depending on the hematocrit. To simulate the effect of laser learning on the model blood layer under study to obtain an opto-acoustic response from single red blood cells and a group of erythrocytes. It was believed that the cells were exposed to a laser with a constant intensity, regardless of their orientation. The laser parameters were chosen for the existing LIMO 100-532/1064-U installation, the Nd: YAG laser, in order to further verify the simulation results by experiment. The result of the simulation proves the possibility of counting the number of red blood cells and measuring the hematocrit of un aggregated erythrocytes, by recording the signal amplitude and measuring the spectral power. The registered parameters grow with the increase in the number of erythrocytes, and the frequency of the signal spectrum also changes, which also informs about the quantitative composition of red blood cells. The work was carried out at the Southern Federal University and is a continuation of the study on the development of a flow optical cytometer. Keywords: optoacoustics, laser, power spectral density, optoacoustic signals, erythrocytes, hematocrit, signal spectrum.