goslasmedЛазерная медицинаLaser Medicine2071-80042686-8644Skobelkin Centre for Laser Medicine - a branch of the Federal Clinical Center for High Medical Technologies, FMBA of Russia10.37895/2071-8004-2015-19-3-46-56goslasmed-41Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯORIGINAL RESEARCHESВлияние непрерывного низкоинтенсивного лазерного излучения красного (635 нм) и зеленого (525 нм) спектров на мезенхимальные стволовые клетки человека in vitroEffects of continuous low-intensive laser irradiation with red (635 nm) and green (525 nm) light of human mesenchymal stem cells in vitroМосквинСергей ВладимировичMoskvinS. V.7652612@mail.ruКлючниковД. Ю.KljuchnikovD. Yu.noemail@neicon.ruАнтиповЕ. В.AntipovE. V.noemail@neicon.ruГоринаА. И.GorinaA. I.noemail@neicon.ruКиселеваО. Н.KiselevaO. N.noemail@neicon.ruФГБУ «Государственный научный центр лазерной медицины ФМБА РФ»РоссияГБУЗ «Самарский областной центр планирования семьи и репродукции»РоссияНОУ ВПО «Медицинский институт «РЕАВИЗ»Россия2015030820181934656Copyright © Москвин С.В., Ключников Д.Ю., Антипов Е.В., Горина А.И., Киселева О.Н., 20182018Москвин С.В., Ключников Д.Ю., Антипов Е.В., Горина А.И., Киселева О.Н.Moskvin S.V., Kljuchnikov D.Y., Antipov E.V., Gorina A.I., Kiseleva O.N.This work is licensed under a Creative Commons Attribution 4.0 License.https://goslasmed.elpub.ru/jour/article/view/41

Введение. Одним из способов неспецифического регулирования клеточной активности МСК на этапе предварительного культивирования in vitro является воздействие низкоинтенсивным лазерным излучением (НИЛИ). В задачи исследования входило изучение предельных режимов непрерывного НИЛИ красного (635 нм) и зеленого (525 нм) спектров. Материалы и методы. В эксперименте использовали адгезивную культуру МСК, 4 пассажа, полученных из ткани пуповины донора, давшего информированное согласие. Для освечивания использовали лазерный терапевтический аппарат «Лазмик-ВЛОК» (РУ № РЗН 2014/1410 от 06.02.2014), лазерные излучающие головки с одним лазерным диодом КЛО-635-40 (635 нм, 4,9 мВт/см2) и КЛО-525-50 (525 нм, 5,4 мВт/см2), работающие в непрерывном режиме. Фиксация лазерных излучающих головок обеспечивалась специальной насадкой (банкой) для лазерно-вакуумного массажа из комплекта КБ-5 диаметром 35 мм. Экспозиция во всех режимах составляла 5 мин. Выводы. Показано, что при используемых энергетических и временных параметрах воздействия морфология и жизнеспособность МСК не меняется. Полученные нами данные демонстрируют предельный уровень выбранных энергетических параметров, при которых наблюдается не просто отсутствие положительного эффекта в отношении стимулирования пролиферации, но возможно, и ингибирование.

Introduction. One of the techniques for non-specific regulation of cellular activity in mesenchymal stem cells (MSC) at the stage of preliminary cultivation in vitro is irradiation with low-intensive laser light (LILI). The aim of the present work was to study limiting regimes for continuous LILI of red (635 nm) and green (525 nm) spectra. Materials and methods. The adhesive MSC culture, 4 passages, received from the umbilical tissue of a donor who had given his informed consent was used in the study. Irradiation was done with laser therapeutic device «Lazmik-VLOK» (RU No RZN 2014/1410 dated 06.02.2014) having emitting heads with one laser diode KLO-635-40 (635 nm, 4.9 mWt/cm2) and KLO-525-50 (525 nm, 5.4 mWt/сш2) in continuous mode. The laser emitting heads were fixed with a special attachment for laser-vacuum massage taken from the set KB-5, diameter 35 mm. Exposure time in all regimes was 5 min. Conclusion. It has been shown that morphology and MSC vital capacity do not change under the applied energetic and time parameters. The obtained findings have defined limit values of chosen energetic parameters under which there is not only lack of positive effect as to the proliferation stimulation but, possibly, its inhibition.

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The authors declare that there are no conflicts of interest present.